Dr. John P. Marino

Dr. John P. Marino

Positions:

Research Chemist, NIST

Adjunct Associate Professor

Education:

Ph.D. Chemistry, Yale University, 1995

A.B. Chemistry, Princeton University, 1989

Email: marino@umbi.umd.edu

Homepage: Marino Lab

Voice:(240) 314-6160

FAX:(240) 314-6255
Mailing info CARB Faculty Directory

Research Overview

Nuclear Magnetic Resonance Spectroscopy (NMR)

NMR is a technique for determining molecular structure, and can also be used to examine the changes that take place when molecules interact or bind to one another. The technique applies powerful external magnetic fields to align the nuclei of certain atoms and then measures the responses of those atoms when they are disturbed by electromagnetic radiation. Trained specialists can use the data gathered to deduce the precise structure and arrangement of...

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Research Description

Research Area: Structural Biology

Research Specialties: RNA Structure and RNA-Protein Interactions, Targeting RNA with Small Molecules, Biomolecular NMR Methodology, Single Molecule Studies of RNA Complexes

Our research focuses on applying high-resolution nuclear magnetic resonance (NMR), together with other biophysical techniques, to study macromolecular structure, interactions and function. Current projects in the laboratory aim to understand the role of RNA/DNA structures and interactions in plasmid copy number regulation and retroviral replication and the structural basis for guanine nucleotide exchange in G Protein Coupled Receptor signaling. To advance our studies, we are involved in developing new NMR and fluorescence methods for characterizing macromolecular structure, dynamics and interactions.

Additional Info

National Institute of Standards and Technology (NIST)

Chemical Science and Technology Laboratory (CSTL)

Biochemical Division

Representative Publications

Ridge KD, J. P. Marino, T. Ngo, E. Ramon, D. M. Brabazon, and N. G. Abdulaev (2006) NMR Analysis of Rhodopsin-Transducin Interactions. Journal of Vision Research, in press.

Ridge KD, Abdulaev NG, Zhang C, Ngo T, Brabazon DM, Marino JP. (2006) Conformational changes associated with receptor stimulated guanine nucleotide exchange in a heterotrimeric G-protein alpha -subunit: NMR analysis of GTP?S-bound states. J. Biol. Chem. 2006 Jan 9; [Epub ahead of print]

Dash C, Marino JP, Le Grice SF. (2006) Examining Ty3 polypurine tract structure and function by nucleoside analog interference J. Biol. Chem., 281(5):2773-83. Epub 2005 Nov 22.

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